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A common obstacle faced when attempting to extract DNA and RNA from cells of interest is the release of denaturing enzymes like DNase and RNase during cell lysis. Guanidine Thiocyanate is a cost effective chemical tool that prevents these nucleases from degrading precious nucleic acids by irreversibly inhibiting their catalytic activity.
Guanidine Thiocyanate also has the added benefit of being a chaotropic agent. This feature allows it to decrease the action of hydrogen bonds in water as well as disrupts intramolecular interactions within proteins and other macromolecules – further increasing solubility of unwanted cellular components.
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