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Assay type | Sandwich-ELISA |
Format | 96T/48T/24 |
Assay time | 1.5h |
Detection range | 125.00-4000 pg/mL |
Sensitivity | 53.10 pg/mL |
Sample type &Sample volume | serum and plasma; 50μL |
Specificity | This kit recognizes D2D in samples. No significant cross-reactivity or interference between D2D and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of D2D concentrations in serum and plasma. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T | 2-8℃, 1 months |
Reference Standard | 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials | 2-8℃, use the reconstituted standard within 24h |
Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL | 2-8℃(Protect from light) |
Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL | 2-8℃ |
HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL | |
Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL | |
Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL | 2-8℃ |
Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces | |
Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level D2D were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level D2D were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 193.00 | 640.27 | 1476.29 | 177.05 | 587.91 | 1461.37 |
Standard deviation | 11.04 | 32.91 | 53.74 | 8.89 | 23.69 | 47.64 |
CV (%) | 5.72 | 5.14 | 3.64 | 5.02 | 4.03 | 3.26 |
The recovery of D2D spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 93-108 | 100 |
EDTA plasma(n=8) | 90-104 | 96 |
Synonyms | D-Dimer,D dimer,D2D |
Research Area | Cardiovascular |
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